Fermentation of 10% (w/v) Sugar to D(−)-Lactate by Engineered Escherichia coli B
AbstractDerivatives of ethanologenic Escherichia coli K011 were constructed for d(−)-lactate production by deleting genes encoding competing pathways followed by metabolic evolution, a growth-based...
View ArticleFermentation of 12% (w/v) Glucose to 1.2 m Lactate by Escherichia coli Strain...
AbstractA non-recombinant mutant of Escherichia coli B, strain SZ194, was developed that produces over 1 md-lactate from glucose (or sucrose) in 72 h using mineral salts medium supplemented with 1 mm...
View ArticleBetaine Tripled the Volumetric Productivity of d(-)-lactate by Escherichia...
AbstractOsmotic stress restricts glycolytic flux, growth (rate and yield), d-lactate productivity, and d-lactate tolerance in Escherichia coli B strain SZ132 during batch fermentation in mineral salts...
View ArticleMethylglyoxal Bypass Identified as Source of Chiral Contamination in l(+) and...
AbstractTwo new strains of Escherichia coli B were engineered for the production of lactate with no detectable chiral impurity. All chiral impurities were eliminated by deleting the synthase gene...
View ArticleLow salt medium for lactate and ethanol production by recombinant Escherichia...
AbstractIndividual nutrient salts were experimentally varied to determine the minimum requirements for efficient l(+)-lactate production by recombinant strains of Escherichia coli B. Based on these...
View ArticleProduction of l-alanine by metabolically engineered Escherichia coli
AbstractEscherichia coli W was genetically engineered to produce l-alanine as the primary fermentation product from sugars by replacing the native d-lactate dehydrogenase of E. coli SZ194 with alanine...
View ArticleRe-engineering Escherichia coli for ethanol production
AbstractA lactate producing derivative of Escherichia coli KO11, strain SZ110, was re-engineered for ethanol production by deleting genes encoding all fermentative routes for NADH and randomly...
View ArticleEngineering Escherichia coli for Fermentative Dihydrogen Production:...
AbstractTrichomonas vaginalis generates reduced ferredoxin within a unique subcellular organelle, hydrogenosome that is used as a reductant for H2 production. Pyruvate ferredoxin oxidoreductase and...
View ArticleThermophilic Bacillus coagulans Requires Less Cellulases for Simultaneous...
AbstractEthanol production from lignocellulosic biomass depends on simultaneous saccharification of cellulose to glucose by fungal cellulases and fermentation of glucose to ethanol by microbial...
View ArticleDeletion of methylglyoxal synthase gene (mgsA) increased sugar co-metabolism...
AbstractThe use of lignocellulose as a source of sugars for bioproducts requires the development of biocatalysts that maximize product yields by fermenting mixtures of hexose and pentose sugars to...
View ArticlePhysiological and fermentation properties of Bacillus coagulans and a mutant...
AbstractBacillus coagulans, a sporogenic lactic acid bacterium, grows optimally at 50–55°C and produces lactic acid as the primary fermentation product from both hexoses and pentoses. The amount of...
View ArticleYqhC regulates transcription of the adjacent Escherichia coli genes yqhD and...
AbstractPrevious results have demonstrated that the silencing of adjacent genes encoding NADPH-dependent furfural oxidoreductases (yqhD dkgA) is responsible for increased furfural tolerance in an E....
View Articlel(+)-Lactic acid production from non-food carbohydrates by thermotolerant...
AbstractLactic acid is used as an additive in foods, pharmaceuticals, and cosmetics, and is also an industrial chemical. Optically pure lactic acid is increasingly used as a renewable bio-based product...
View ArticleComplete Genome Sequence of a thermotolerant sporogenic lactic acid...
AbstractBacillus coagulans is a ubiquitous soil bacterium that grows at 50–55 °C and pH 5.0 and ferments various sugars that constitute plant biomass to L (+)-lactic acid. The ability of this...
View ArticleOptical mapping and sequencing of the Escherichia coli KO11 genome reveal...
AbstractEscherichia coli KO11 (ATCC 55124) was engineered in 1990 to produce ethanol by chromosomal insertion of the Zymomonas mobilispdc and adhB genes into E. coli W (ATCC 9637). KO11FL, our current...
View ArticleRemoving chiral contamination of lactate solutions by selective metabolism of...
AbstractObjectiveA bio-based process is appealing for purification of l-lactic acid, the major enantiomer of polylactic acid syrup, generated by thermochemical processes at the end of life of PLA-based...
View ArticleSweet Sorghum Juice and Bagasse as Feedstocks for the Production of Optically...
AbstractSweet sorghum is a bioenergy crop that produces large amounts of soluble sugars in its stems (3–7 Mg ha−1) and generates significant amounts of bagasse (15–20 Mg ha−1) as a lignocellulosic...
View ArticleTechno-Economic Evaluation of Cellulosic Ethanol Production Based on Pilot...
AbstractReplacing fossil fuels with renewable fuels derived from lignocellulosic biomass can contribute to the mitigation of global warming and the economic development of rural communities. This will...
View ArticleMetabolic engineering of Escherichia coli for the production of butyric acid...
AbstractBackgroundSeveral anaerobic bacteria produce butyric acid, a commodity chemical with use in chemical, pharmaceutical, food and feed industries, using complex media with acetate as a co-product....
View ArticleA Combined Thermochemical and Microbial Process for Recycling Polylactic Acid...
AbstractPolylactic acid polymer (PLA) produced from renewable resources can be recycled at the end of life to constituent monomer, optically pure lactic acid (LA), by a combination of chemical and...
View Article
